供应水痘带状疱疹病毒
product name: varicella zoster grade 2 antigen
catalog #:ba-na001
agent description: vzv is a large virus with a diameter of about 180-200; its dense core is 100 nm in diameter and is covered by an icosahedral capsid composed of 162 tubular capsomers, in turn covered by a lipid bilayer envelope.
description: the resulting antigen preparation contains a high concentration of virus and viral components as well as some cellular material suspended in emem with some serum proteins.
inactivation: varicella zoster antigen is inactivated using gamma radiation. this procedure is effective primarily by damaging viral genetic matrial.
cultured in: mrc-5
strain: vz-10
buffer: emem
applications: widely used for both igg and igm detection in assays which include eia with polystyrene and latex solid phases.
preparation: optimally infected monolayers are harvested, resuspended in a small volume of tissue culture fluid and disrupted by sonication.
storage: store at -70℃ to -100℃. repeated freezing and thawing should be avoided.
hazards: inactivated and should contain no infectious material. generally accepted good laboratory practices appropriate to biological should be employed when handling this product.
recommendations for use: the antigen should be sonicated immediately prior to use to ensure that the preparation is uniform. this preparation may be used as is in a variety of immunoassay formats or may be further purified to meet the needs of a particular assay format.
quality control information
product name: varicella zoster grade 2 antigen
bioar performs quality control tests to ensure each batch meets in-horse specification. it is important that each user perform titrations of antigen using their own assay as each assay format and serum release panel makes different performance demands on the antigen.
test:
titre: this antigen preparation is titrated using a microtitre plate based elisa. antigens are tested for reactivity with igg. the dilution of antigen which generates a singal of 1.0 o.d unit in the immunoassay is compared to that of the standard approved antigen. the result of this comparison is expressed as a percentage of the reference.
igg result: 173.6% of reference ag
protein concentration: protein is determined using the dye binding assay in the microassay format. the standard curve is generated with a known concentration of igg.
rusult: 1.48 mg/ml
inativation assay: the effectiveness of inactivation is tested by inoculating a mrc-5 monolayer with antigen.
result: no growth detected
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